Research Article
Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR
Table 7
Final ranking of HKGs.
| | Algorithm | GSC | dGSC | ASC |
| | ΔCt comparative method | SDHA/ACTB/B2M | TBP/SDHA/ALAS1 | SDHA/HPRT1 |
| | GeNorm | SDHA/ACTB/UBC | TBP/ALAS1/SDHA | GAPDH/B2M | | SDHA/B2M/ACTB | TBP/SDHA/ALAS1 | HPRT1/SDHA | | 3 genes | 3 genes | 2 genes |
| | BestKeeper | SDHA/B2M/ACTB | B2M/SDHA/HPRT1 | HPRT1/SDHA |
| | NormFinder | SDHA/B2M/ACTB | TBP/SDHA/ALAS1 | SDHA/TBP/RPS18 |
| | RefFinder | UBC/ACTB/SDHA | TBP/SDHA/ACTB | HPRT1/SDHA |
| | Selected genes | SDHA/ACTB/B2M | TBP/SDHA | HPRT1/SDHA |
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