Osteogenic Differentiation of Human Amniotic Fluid Mesenchymal Stem Cells Is Determined by Epigenetic Changes

Table 1

Gene	Primer sequence (5′-3′)	Product size (bp)
Oct4	Forward 5′-CGAGAAGGATGTGGTCCGAG	136
	Reverse 5′-CAGAGGAAAGGACACTGGTC
Nanog	Forward 5′-AGATGCCTCACACGGAGACT	96
	Reverse 5′-GTTTGCCTTTGGGACTGGTG
Sox2	Forward 5′-TGGACAGTTACGCGCACAT	215
	Reverse 5′-CGAGTAGGACATGCTGTAGGT
Rex1	Forward 5′-GCCTTATGTGATGGCTATGTGT	96
	Reverse 5′-ACCCCTTATGACGCATTCTATGT
Alkaline phosphatase (ALP)	Forward 5′-AGCCCTTCACTGCCATCCTGT	68
	Reverse 5′-ATTCTCTCGTTCACCGCCCAC
Osteopontin	Forward 5′-GAAGTTTCGCAGACCTGACAT	91
	Reverse 5′-GTATGCACCATTCAACTCCTCG
GAPDH	Forward 5′-AGTCCCTGCCACACTCAG	123
	Reverse 5′-TACTTTATTGATGGTACATGACAAGG