BMP6-Engineered MSCs Induce Vertebral Bone Repair in a Pig Model: A Pilot Study

Figure 2

pBM-MSCs nucleofected with rhBMP-6 secrete BMP6 in vitro. Porcine BM-MSCs were nucleofected with cDNA3-pCMV-rhBMP-6 and cultured in vitro. The media were changed after 24 hours and 48 hours after nucleofection the secretion of the BMP6 protein was evaluated using quantitative protein immunoassay (ELISA). The amount of BMP6 secreted was normalized to cell number and compared to the cells that were nucleofected with GFP; bars indicate SE, .