Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method

Figure 2

Effect of various experimental variables on UC-MSCs isolation. (a) The new methods average cell number per cm of umbilical cord isolated, compared to the old cell number isolated per cm ( means ). (b) Comparing different experimental variables. Significant difference observed in Caesarean-section delivery versus vaginal delivery ( means ). (c) Population doubling time for passage 0 (initial isolation) or passage 1 (first passage of expansion phase). represents for 2% hpl media compared to 5% and 10% media. † represents for the passages (P0 compared to the P1).

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