Characterization of Neurogenic Potential of Dental Pulp Stem Cells Cultured in Xeno/Serum-Free Condition: In Vitro and In Vivo Assessment

Figure 3

Comparison of marker expressions between FBS group (FBS, white bar) and NSC group (NSC, black bar) at passage 5. qRT-PCR was applied for measurement of NSC or neuronal markers (Dcx, Map2, Mash1, NCAD, NCAM, nestin, NeuroD1, Olig2, Pax6, Sox1, and Sox2), MSC marker (fibronectin), and both NSC and MSC markers (vimentin) in the FBS and NSC groups. The results are displayed relative to the expressions in FBS medium cells via calculation using the ΔΔCT and 2-(ΔΔCT) methods and normalized to 18s ribosomal RNA expression, and the error bars represent the standard deviation of mean from three donors in each group (). The asterisk () indicates significant difference in comparison with the FBS group according to the independent -test ().