PTF1a Activity in Enriched Posterior Foregut Endoderm, but Not Definitive Endoderm, Leads to Enhanced Pancreatic Differentiation in an In Vitro Mouse ESC-Based Model

Figure 3

Analysis of mESC-derived posterior foregut endoderm. (a) Posterior foregut endoderm markers, Hlxb9, Hnf6, Hnf1b, and Pdx1, were significantly higher in Nodal- and Activin-derived cultures than in no growth factor treated (No-GF) control cultures. On the other hand, Hnf4a that is expressed in liver progenitors was reduced. -3. Data are presented as mean ± SEM. Asterisks indicate values on comparison with No-GF control cultures: , , and determined by one-way ANOVA with Tukey’s multiple comparison test. (b) Immunofluorescent staining of Nodal-derived posterior foregut endoderm cultures indicates the expression of the above-mentioned markers at the single-cell protein level. Pdx1 is expressed in Hnf6-expressing cells. Scale bars, 100 μm.

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