Real-Time Analysis of Endogenous Wnt Signalling in 3D Mesenchymal Stromal Cells

Figure 2

Endogenous Wnt signalling is not activated during 2D MSC differentiation. (a) Percentage of positively expressing human Y201 MSC-EGFP reporter cells during osteogenic differentiation as measured by flow cytometry, versus basal control with representative flow cytometry plot. (b) Alizarin red staining for calcium deposition during osteogenic differentiation. Scale bar = 200 μm. (c) Percentage of positively expressing human Y201 MSC-EGFP cells during adipogenic differentiation as measured by flow cytometry, versus basal control with representative flow cytometry plot. (d) Oil Red O staining for lipid droplet formation during adipogenic differentiation. Scale bar = 200 μm.

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