Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro

Table 1

Primers used for qPCR analysis.

Gene (protein)	Forward primer sequences	Reverse primer sequences	qPCR efficiency	Length of amplicon
Homo sapiens
Kit (c-kit)	5′-CTTCCTTATGATCACAAATGGGAGTT-3′	5′-TCCAGCACCCAGGGTTTTC-3′	98%	65 nt
Mef2c (MEF2C)	5′-CCATCTGCCCTCAGTCAGTTG-3′	5′-AGGCAGGGAGAGATTTGAACTCT-3′	100%	72 nt
Gata4 (GATA-4)	5′-AGCCTGGCCTGTCATCTCACTA-3′	5′-GGCCAGACATCGCACTGACT-3′	100%	73 nt
Polr2b (POLR2B)	5′-CCTGATCATAACCAGTCCCCTAGA-3′	5′-GTAAACTCCCATAGCCTGCTTACC-3′	96%	69 nt