Umbilical Cord Tissue-Derived Mesenchymal Stem Cells Induce T Lymphocyte Apoptosis and Cell Cycle Arrest by Expression of Indoleamine 2, 3-Dioxygenase

Figure 3

IDO, expressed by hUC-MSCs stimulated by IFN-γ in culture, was associated with the suppression of T lymphocyte proliferation. (a) T lymphocytes were stimulated with PHA (10 μg/mL) during coculture with hUC-MSCs. T cell proliferation was measured by Brdu incorporation at 120 h. Results are the average of 3 independent experiments. , compared to coculture system that added 1-MT. (b) hUC-MSCs which were cocultured with T lymphocytes expressed protein level of IDO. (c) IFN-γ stimulated indeed the expression of IDO in UC-MSCs. (d) IDO activity was quantified in T lymphocytes or cocultures. Results are the average of 3 independent experiments. , compared to coculture system that added 1-MT.

(a)

(b)

(c)

(d)