Role of MSX1 in Osteogenic Differentiation of Human Dental Pulp Stem Cells

Table 1

Primer and probe sequences used for RT-qPCR.

Gene	Primer ()	Probe
MSX1 (sense)	F: CTCGTCAAAGCCGAGAGC	Roche Universal Probe # 7
	R: CGGTTCGTCTTGTGTTTGC
MSX1 (antisense)	F: GCCAGCCCTCTTAGAAACAG	Roche Universal Probe # 50
	R: AATAAAGCAGCCCCTCGTTC
RUNX2	F: CAGTGACACCATGTCAGCAA	Roche Universal Probe # 66
	R: GCTCACGTCGCTCATTTTG
BMP2	F: CGGACTGCGGTCTCCTAA	Roche Universal Probe # 49
	R: GGAAGCAGCAACGCTAGAAG
OSX	F: CAGCAGCTAAACTTGGAAGGA	Roche Universal Probe # 76
	R: TGCTTTCGCTTGTCTGAGTC
OCN	F: GCCTCCTGAAAGCCGATGT	5′-CCAACTCGTCACAGTCCGGATTGAGCT-3′
	R: AAGAGACCCAGGCGCTACCT
ALPL	F: TCACTCTCCGAGATGGTGGT	Roche Universal Probe # 12
	R: GTGCCCGTGGTCAATTCT
SOX9	F: GTACCCGCACTTGCACAAC	Roche Universal Probe # 61
	R: TCTCGCTCTCGTTCAGAAGTC
PPAR	F: GACAGGAAAGACAACAGACAAATC	Roche Universal Probe # 7
	R: GGGGTGATGTGTTTGAACTTG
SREBP2	F: GCCCTGGAAGTGACAGAGAG	Roche Universal Probe # 21
	R: TGCTTTCCCAGGGAGTGA
HMGCS1	F: TCTGTCTACTGCAAAAAGATCCAT	Roche Universal Probe # 59
	R: TGAAGCCAAAATCATTCAAGG
HMGCR	F: GTTCGGTGGCCTCTAGTGAG	Roche Universal Probe # 65
	R: GCATTCGAAAAAGTCTTGACAAC
FDPS	F: GGCCACTCCAGAACAGTACC	Roche Universal Probe # 75
	R: CCTCATATAGCGCCTTCACC
CYP51A1	F: TGCAGATTTGGATGGAGGTT	Roche Universal Probe # 64
	R: CCTTGATTTCCCGATGAGC
DHCR7	F: GCCATGGTCAAGGGCTAC	Roche Universal Probe # 60
	R: TTGTAAAAGAAATTGCCTGTGAAT

MSX1: msh homeobox 1; RUNX2: runt-related transcription factor-2; BMP2: bone morphogenetic protein-2; OSX: osterix; OCN: osteocalcin; ALPL: alkaline phosphatase liver type; SOX9: SRY- (sex determining region Y-) box 9; PPAR: peroxisome proliferator activated receptor gamma; SREBP2: sterol regulatory element-binding protein 2; HMGCS1: 3-hydroxy-3-methylglutaryl-CoA synthase 1; HMGCR: HMG-CoA reductase; FDPS: farnesyl diphosphate synthase; CYP51A1: Cytochrome P450 Family 51 Subfamily A Polypeptide 1; DHCR7: 7-dehydrocholesterol reductase; F: forward; R: reverse.