Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity

Figure 3

Expansion of Pvec⁺ cells. (a-b) Schematic representation of expansion strategy and propagation of Pvec⁺ cells. Addition of GSK3 inhibitor (SB-216763) induces propagation of Pvec⁺ cells expressing Isl1 at d12. (c) Pvec⁺/Isl1⁺ cells percentage in SB-216763 from three independent experiments. For each experiment ~1000 cells were counted. (d) Pvec⁺ cell growth curve. (e–j) Propagated cells consist of cell populations expressing markers of cardiac (Isl1, Mef2c, and GATA4) and/or endothelial (Flk-1, PECAM-1, and VE-cadherin) progenitors.

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