iPSC-Derived Retinal Pigment Epithelium Allografts Do Not Elicit Detrimental Effects in Rats: A Follow-Up Study

Figure 3

Data from immunocytochemistry and flow cytometry-based phagocytosis assays further suggest that the pigmented cells in the SRS are human RPE. (a and b) Pigmented clumps of cells from the subretinal space were isolated using RPE primary culture techniques (a). Some of the isolated cells are human RPE (labeled with antibodies that only recognize human OTX2—a molecular marker for RPE; DAPI = red; OTX2 = green (b)). (c) Some of the isolated cells internalized FITC-labeled porcine outer segments after five hours (labeled as phagocytes; green) in histograms (top panels) and scatter plots (bottom panels). DRAQ5 is a nuclear marker for living cells. Scale bars = 100 μm.

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