Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells

Figure 8

Immunohistochemistry of hFibs, hESCs, and haGSCs with cytoplasmic staining of VASA ((a)–(c)), nuclear staining of UTF1 ((d)–(f)), cytoplasmic staining of TSPYL2 ((g)–(i)), cytoplasmic and nuclear staining of STELLA ((j)–(l)), and cytoplasmic staining of GFRα1 ((m)–(o)). The different germ cell different markers are shown in green (Alexa 488 for TSPYL2 and GFRα1) or red (Alexa 546 for VASA, UTF1, and STELLA) and the staining of the nuclei with HOECHST is shown in blue. Scale bar is 50 μm.