Differentiation and Molecular Properties of Mesenchymal Stem Cells Derived from Murine Induced Pluripotent Stem Cells Derived on Gelatin or Collagen

Figure 4

Osteogenic and adipose differentiation of iMSC/G and C derived from an iPSC clone (2A-4F-60). (a) iMSCs cultured in medium containing osteogenic differentiation factors (induction) or absence (control) and stained with Alizarin red at 28 days. (b) Relative amount of Alizarin red-positive areas. value < 0.001. (c) Chondrocyte differentiation of iMSCs by micromass culture. (d) iMSCs cultured in differentiation medium (induction) or absence (control) and stained Oil Red-O at 14 and 21 days. (e) Relative amount of Oil Red-O-positive areas. value < 0.05.

(a)

(b)

(c)

(d)

(e)