Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways

Figure 6

Polydatin protected BMSCs against H₂O₂-induced cell death partly through Nrf 2/ARE pathway. BMSCs were pretreated with polydatin for 2 h and further exposed to H₂O₂ for 12 h. (a) Effects of polydatin on NQO-1 and the phosphorylation of Nrf 2. (b, c) Quantitative analysis of the blots was shown in panel after being normalized by α-tubulin. (d) Cells were treated with different concentration of brusatol for 24 h. Effects of brusatol on phosphorylation of Nrf 2 were detected by Western blot and (g) the bands were normalized by α-tubulin. (e) Cell viability was tested in the presence of different concentration of brusatol. (h) BMSCs were pretreated with brusatol (100 μM) for 1 h followed by incubating with/without polydatin and H₂O₂ for 24 h. (f) ROS production was detected by H2DCF-DA staining. (b) Quantitative analysis of DCF fluorescent intensity. One-way ANOVA followed by Tukey’s test. and versus control group; and versus H₂O₂-treated group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)