HDAC6 Inhibitors Rescued the Defective Axonal Mitochondrial Movement in Motor Neurons Derived from the Induced Pluripotent Stem Cells of Peripheral Neuropathy Patients with HSPB1 Mutation

Figure 4

Reduced acetylation of -tubulin in S135F-MNs and P182-MNs. (a) Acetylation of -tubulin was visualized by immunostaining with anti-acetylated -tubulin Abs (red). Total -tubulin was also detected by staining with anti--tubulin Abs (green) (original magnification, 400x). Scale bars: 100 μm. (b) The extent of acetylated -tubulin was normalized with corresponding total -tubulin using ImageJ software (WA09-MNs; , S135F-MNs; , and P182L-MNs; ). Unpaired -tests, and . (c and d) Western blot assay showed decreased acetylated -tubulin levels in S135F-MNs and P182-MNs (paired -tests, and ).

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