Effects of FTY720 (Fingolimod) on Proliferation, Differentiation, and Migration of Brain-Derived Neural Stem Cells

Figure 1

Identification of primary cultured NSCs in vitro. (a) Representative neurospheres in culture. ((b)–(d)) Immunocytochemical staining of purified NSCs with Nestin ((b), green) and Brd-U ((c), red) and merged with nucleus staining DAPI ((d), blue) (bar = 160 μm). ((e)–(h)) Nucleus staining ((e), blue) and immunocytochemical of differentiated astrocytes with β-tubulin III ((f), green) and GFAP ((g), red) and merged with (h) (bar = 80 μm).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)