Effects of FTY720 (Fingolimod) on Proliferation, Differentiation, and Migration of Brain-Derived Neural Stem Cells

Figure 3

Differentiation of NSCs into neurons and astrocytes in the presence of FTY720. (a) Representative images of neurons (β-tubulin III positive, in green) and astrocytes (GFAP positive, in red) in different groups. Bar = 80 μm. Arrows indicate protoplasmic astrocytes. ((b)-(c)) Quantification of the proportion of differentiated neurons and astrocytes. . ((d)-(e)) Western blot analysis of β-tubulin III protein in different groups. in each group. ((f)-(g)) Western blot analysis of GFAP protein in different groups. in each group. compared with control group; compared with 1 nM FTY720 group, by ANOVA followed by Dunnett’s multiple comparison tests.

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