IL-1/TNF-α Inflammatory and Anti-Inflammatory Synchronization Affects Gingival Stem/Progenitor Cells’ Regenerative Attributes

Figure 1

Isolation and characterization of G-MSCs. (a) Microscopic appearance of outgrowing cells from free gingival margin. (b) Microscopic appearance of G-MSCs stained with crystal violet. (c) Alizarin red staining of G-MSCs after osteogenic induction. (d) Alizarin red staining of G-MSCs cultured in a basic medium. (e) Oil Red O staining of G-MSCs after adipogenic stimulation. (f) Oil Red O staining of G-MSCs cultured in a basic medium. (g) Alcian blue staining of G-MSCS after chondrogenic stimulation. (h) Alcian blue staining of G-MSCs cultured in a basic medium. (i) Flow cytometry analysis of cell surface markers of G-MSCs.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)