Periodontal Ligament Stem Cells in the Periodontitis Microenvironment Are Sensitive to Static Mechanical Strain

Figure 1

The cell culture and identification of HPDLSCs and PPDLSCs. (a) Morphologies of HPDLSCs observed by microscopy. (b) Morphologies of PPDLSCs observed by microscopy. (c) Mesenchymal stem cell phenotype examination of HPDLSCs by flow cytometric analysis. (d) Mesenchymal stem cell phenotype examination of PPDLSCs by flow cytometric analysis. (e) Quantitative data for mesenchymal stem cell phenotype examination of HPDLSCs and PPDLSCs. (f) Quantitative analysis of mineralized nodule by calcium level analysis. (g). Quantitative analysis of lipids by isopropanol dissolution and absorbance measurement. versus matched PPDLSCs. Scale bar = 100 μm; in each group. Each experiment was performed three times. The data are presented as the mean ± standard deviation.

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