Stemness Characteristics of Periodontal Ligament Stem Cells from Donors and Multiple Sclerosis Patients: A Comparative Study

Figure 8

Senescence markers p16 and p21 expression. Immunofluorescence staining of p16 in P2 and P15 hPDLSCs ((a) and (b), resp.) and p21 in P2 and P15 hPDLSCs ((d) and (e), resp.). Histograms show relative fold changes of qRT-PCR for p16 (c) and p21 (f) in hPDLSCs. Immunofluorescence staining of p16 in P2 and P15 MS-hPDLSCs ((g) and (h), resp.) and p21 in P2 and P15 MS-hPDLSCs ((j) and (k), resp.). Histograms show relative fold changes of qRT-PCR for p16 (i) and p21 (l) in MS-hPDLSCs. Green, red, and blue fluorescence was applied to stain actin cytoskeleton, p16 or p21, and nuclei, respectively. Mag: 63x. Bar: 20 μm. value < 0.05. Data are expressed as mean ± SEM.