Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization

Figure 3

Fluorescence staining of hydroxyapatite and PCA of Raman spectra from differentiated MSCA-1⁺ JPCs. (a, b, d, e, g, h) OsteoImage fluorescence staining and (c, f, i) PCA scores plots of JPCs isolated from (a–c) patient #1, (d–f) patient #2, and (g–i) patient #3 after differentiation in (a, d, g) serum-containing DMEM and under (b, e, h) serum-free conditions (MC) for 20 days. Scale bar equals 200 µm. (c, f, i) PCA plots of Raman spectra using PCs shown in Figure 4. The PCAs reflect a more mature bone formation (green ellipse) under MC media conditions compared to DMEM conditions (yellow ellipse) after 20 days of differentiation for all patients.

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