Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Figure 11

Effect of TGF-β1 inhibitor on the expression of catabolic proteins and genes on chondrocytes cultured in different conditions for 5 days determined by western blot ((a), (b), (d), and (e)) and real-time PCR ((c), (f)). Both western blot and real-time PCR showed that some catabolic marker proteins and genes such as caspase-3, MMP3, and MMP13 were downregulated in chondrocytes when they were cultured with ADSCs coculture system and recovered back to the original levels when TGF-β1 inhibitor was added in chondrocytes cocultured with ADSCs as tested by western blot ((a), (b), (d), and (e)) and real-time PCR ((c), (f)). Data are shown as mean ± SEM. versus control group. means versus chondrocytes.

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