Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Figure 4

Proliferation of chondrocytes cultured with three different conditions for 5 days. Morphology of chondrocytes culture with a control medium (a), ADSCs coculture condition (b) and TGF-β1-containing medium (c). Growth curves of chondrocytes cultured in three different conditions are shown ((d), (e)). Chondrocytes: chondrocytes were cultured in normal culture medium. Coculture: chondrocytes were cocultured with ADSCs. TGF-β1: chondrocytes were cultured with TGF-β1-containing medium. All were determined by MTT assay. Cyclin D1 protein expression in chondrocytes cultured in three different conditions for 5 days as determined by western blot (f). Gene expression of Cyclin D1 in chondrocytes cultured in three different conditions for 5 days as determined by real-time PCR. Data are shown as mean ± SEM. versus control group (chondrocytes).

(a)

(b)

(c)

(d)

(e)

(f)

(g)