Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes

Figure 5

Chondrogenesis marker expression in chondrocytes cultured with three different conditions for 9 days detected by histochemical staining ((a)–(i)) and ELISA (j). Alcian blue staining is shown in ((a)–(c)). Toluidine blue staining is shown in ((d)–(f)). Safranin O staining is shown in ((g)–(i)). Chondrocytes were cultured in control medium ((a), (d), and (g)), or ADSCs cocultured system ((b), (e), and (h)), or TGF-β1-containing medium ((c), (f), and (i)) and stained by the above three markers. The production of glycosaminoglycan (GAG) was analyzed using ELISA kit (j). Data are shown as mean ± SEM. versus control group. Control: chondrocytes were cultured in normal culture medium. Coculture: chondrocytes were cocultured with ADSCs together. TGF-β1: chondrocytes were cultured with TGF-β1-containing medium.

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