Comparing Circadian Dynamics in Primary Derived Stem Cells from Different Sources of Human Adult Tissue

Figure 4

Clock gene expression in human adult stem cells following mechanical rhythmic stimulation paradigm. (a) Schematic diagram showing the setup of the mechanical stretch apparatus; cells were seeded into silicone chambers and stretched for 3 days using a unique uniaxial stretch rig with offset cams (1 Hz, 6.66% stretch, 12 h ON (stretch), 12 h OFF (rest)). (b) Quantitative RT-PCR analyses showing the expression levels of clock genes at two opposite circadian phases (20 h versus 32 h) following rhythmic mechanical stimulation protocol. Data are expressed as the mean of ΔCt ± SEM relative to the 20 h time point and normalised against the housekeeping gene GAPDH. Bars represent means ± SEM of 3 independent experiments, (independent t-test).

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