Comparing Circadian Dynamics in Primary Derived Stem Cells from Different Sources of Human Adult Tissue

Figure 6

Human pluripotency marker SOX2 shows cyclical temporal gene expression in adult stem cells initially following dexamethasone or rhythmic mechanical stimulation. Quantitative RT-PCR analyses showing temporal expression profile of the pluripotency marker Sox2 synchronisation with dexamethasone or rhythmic mechanical stimulation and collected every four hours over 1 circadian cycle (16 h–36 h). Data are expressed as the mean of ΔCt ± SEM relative to 16 h time point and normalised against the housekeeping gene GAPDH. Bars represent means ± SEM of 3 independent experiments.