Insulin-Like Growth Factor Binding Protein-6 Alters Skeletal Muscle Differentiation of Human Mesenchymal Stem Cells

Figure 2

PMSCs cultured under muscle differentiation conditions increased levels of muscle markers (Pax3/7, MyoD, MyoG, and MHC) and decreased OCT4 and SOX2 levels using Western blots. Compared to PMSCs cultured under control conditions, (a) OCT4 was decreased at 1 and 14 days under muscle differentiation conditions. (b) SOX2 levels were decreased at each time point under muscle differentiation conditions. (c) Pax3/7 was increased at day 7 and decreased by day 14 under muscle differentiation conditions. (d) MyoD, (e) MyoG, and (f) MHC were increased at each time point under muscle differentiation conditions. Protein levels were quantified by densitometry and normalized to β-actin. Data is presented as the mean ± SEM of 3 independent experiments. Two-way ANOVA with Bonferroni’s multiple comparison test was performed to determine , , and comparing control to muscle differentiation conditions, or ^#, ^##, and ^### comparing the same treatment over time.

(a)

(b)

(c)

(d)

(e)

(f)