The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells

Figure 7

Real-time RT-PCR of DSPP, BSP, and integrins on day 28. hDPSCs (passage number 4) were cultured in different substrate(s) in DMEM supplemented with 10% FBS for five days. Mineralization reagent including β-GP and AA was incorporated on day five. Dexamethasone (100 nM) (D2915, Sigma) was started to be added on day nine. The total culture time was 28 days. DSPP (a), BSP (b) and ITGA1 (c), ITGA4 (d), ITGA7 (e), and ITGB1 (f) mRNA expression was investigated. Different symbols represent significant differences, (except for between noncoated and Npnt-coated in (a); noncoated and BSA-coated and BSA-coated and FCOL1-coated in (b); noncoated and Fn-coated and noncoated and FCOL1-coated in (c); BSA-coated and FCOL1-coated in (d); noncoated and Fn-coated in (e); and noncoated and Npnt-coated in (f)) by post hoc Tukey’s HSD test.

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