Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium

Table 1

Primer sequences for genes designed and used in this study.

Primers	Primer sequences (5′-3′)	Length of production/bp	Notes
LIFR	F: TAGCACGTGAATTGCGGACT	117	RT-PCR & qRT-PCR
	R: CAGTGCAACAACGAATGCGA
Nestin	F: GGAGAAACAGGGCCTACAGAG	112	RT-PCR
	R: TAGGAGGGTCCTGTATGTGGC
GATA-4	F: AATCGAAGACGTCAGCAGGT	123	RT-PCR
	R: GCTCTGTCTTGATGGGACGC
Oct4	F: GTGTTCAGCCAAACGACCATC	143	RT-PCR
	R: GTCTCTGCCTTGCATATCTCC
PDGFRα	F: GTGGAGAATCTGCTGCCTGG	133	RT-PCR & qRT-PCR
	R: TGTAGGTGACGCCGATGTAG
PLZF	F: GCGGAAGACCTGGATGACCT	105	RT-PCR
	R: GTCGTCTGAGGCTTGGATGGT
SOX9	F: GCAAACTCTGGAGACTGCTGAATG	137	RT-PCR
	R: GCCGTTCTTCACCGACTTTCTC
CYP17A1	F: ATTGACTCCAGCATTGGCGA	179	RT-PCR & qRT-PCR
	R: CCGAAGGGCAAGTAGCTCAA
β-actin	F: CTCCATCATGAAGTGCGACGT	114	RT-PCR & qRT-PCR
	R: GTGATCTCCTTCTGCATCCTGTC