Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats

Figure 1

The characterization of injectable hydrogel and rASC viability after seeding. (a) Fabrication of collagen hydrogels. (b) The morphological appearance of CM-Dil-labeled rASCs seeded on collagen hydrogel after 10 days. Scale bar = 20 μm. Green positive represents collagen, red represents CM-Dil-labeled rASCs, and blue represents nuclei. (c) Live/dead cell analysis for ASCs seeded on collagen scaffolds after 10 d seeding. Image displays live cells (green) and dead cells (red). Scale bar = 200 μm. (d) The schematic picture of transplantation. (e) VEGF release in Hyd-CM and Hyd-ASCs on 1 d, 4 d, and 7 d. The experiments were carried out with 4 replicates and data presented as mean ± SD. versus Hyd-CM group.

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