Research Article

Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats

Figure 2

Characterization of rat adipose-derived mesenchymal stem cells (rASCs). (a) Passage 3 of cultured rASCs under light microscope. Representative pictures of (b) adipogenic, (c) osteogenic, and (d) chondrogenic induction for 3 w of rASCs followed by Oil red O, Alizarin red, and Alcian blue staining. Scale bar = 100 μm. (e) Flow cytometry analysis of the cell surface markers CD45, CD31, CD34, CD90.1, CD29, and CD44 expression at passage 3.
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