Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats

Figure 5

M2-CM enhanced EC vasculogenesis in vitro. (a) M2 macrophage induction identification by IF staining for M2 marker Fizz 1. Scale bar = 20 μm. (b) MCP-1, IL 10, and IL 8 released by M2 macrophage. (c) M2-CM promoted EC migration at 24 h through scratch assay. Scale bar = 100 μm. (d) Analysis of relative migration rate of ECs after M2-CM stimulation for 24 h. (e) Typical images of the tube-like structures in the presence of M2-CM cultured on Matrigel at 18 h. Scale bar = 200 μm. (f) Analysis of tube formation counts of different additions of M2-CM. Data were mean ± SEM of three independent experiments. versus CTR group.

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