Rapid Rapamycin-Only Induced Osteogenic Differentiation of Blood-Derived Stem Cells and Their Adhesion to Natural and Artificial Scaffolds

Figure 3

BDSCs osteogenic differentiation with rapamycin and titanium scaffolds. Nuclear and Alizarin Red S stainings on type 1 titanium after differentiation by confocal microscopy analysis ((a) and (b), resp.). Nuclear staining, osteocalcin expression, and merge analysis on type 2 titanium scaffold ((c), (d), and (e), resp.) (osteocalcin green, DAPI blue; (a), (b) bar = 10 mm; (c), (d) 200 μm 4x).

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