Rapid Rapamycin-Only Induced Osteogenic Differentiation of Blood-Derived Stem Cells and Their Adhesion to Natural and Artificial Scaffolds

Figure 6

Analysis to detect BDSC capacity to differentiate on titanium scaffolds. (A) Merge analysis on type 2 titanium scaffold by osteocalcin expression ((B) osteocalcin green) and nuclear staining ((C) DAPI blue) to demonstrate BDSCs osteogenic differentiation. (D) shows a graphical representation of the two channel light intensity signals (expressed in ADG units ×100). It is evident how the blue peaks (nuclear signal) overlap the green peaks (osteocalcin signal). (E) shows time course experiments for osteocalcin expression (expressed as average channel brightness) ((A): bar = 200 μm, (B), (C): bar = 50 μm).