When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells

Figure 3

Application of the lncRNA reporter gene in PSCs. Generation of lncRNA reporter genes can be achieved by (a) knocking in the reporter gene to the lncRNA locus and (b) introducing the lncRNA promoter-driven reporter gene. Application of the lncRNA reporter gene in (c) driving the expression of the transgene (such as the Rosa26 locus). (d) Monitoring the allele-specific gene expression. (e) Isolation of naive ESCs (red dot) (such as the ESRG promoter- or LTR7Y-driven transgene).