Angelica Sinensis Polysaccharide Prevents Hematopoietic Stem Cells Senescence in D-Galactose-Induced Aging Mouse Model

Figure 4

Effect of ASP on β-catenin expression of Sca-1⁺ HSC/HPCs in D-gal- induced aging mice. The Sca-1+ HSC/HPCs were collected after the treatment. (a) The protein expression analyses of β-catenin by Western blot. Proteins were extracted from cellular cytoplasm and nucleus, respectively; β-actin was used as the internal control. (b) The cellular localization of β-catenin by immunofluorescence, PI (red) to visualize nucleus. (c) β-catenin mRNA expression by qRT-PCR, β-actin was used as the internal control. Different letters: .

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