Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2α Improves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model

Figure 1

HIF-2α was overexpressed in rMSCs and rMSCs^HIF-2α showed stem cell characteristics. (a) Representative fluorescence images showing rMSCs transduced with 0, 25, 50, or 100 MOI recombinant lentivirus expressing HIF-2α. The images were collected 48 hours after transduction. (b) The dose of MOI 100 resulted in >80% transduction efficiency. (c) Representative image of Western blot showing HIF-2α expression in rMSCs transduced with different doses of recombinant lentivirus. (d) Representative image of flow cytometry analysis showing abundant expression of CD29 and absence of CD45 on rMSCs^HIF-2α. (e) Alizarin red S staining shows calcium deposit in osteoblasts differentiated from rMSCs^HIF-2α. (f) Adipocytes differentiated from rMSCs^HIF-2α shows positive oil red O staining for lipid droplets. (g) Toluidine blue staining shows positive glycosaminoglycan matrix production from chondrocytes differentiated from rMSCs^HIF-2α.

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