Research Article
Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2α Improves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model
Figure 4
Cell transplantation increased the expression of growth factors and HIF-2α targeting molecules in the muscle tissue of the ischemic limb and cause dynamic change in serum angiopoietin. (a) Representative image of RT-PCR showing that the mRNA levels of VEGF, bFGF, and SDF1 increased and Bax mRNA level reduced 7 days after transplantation of rMSCs or rMSCsHIF-2α. (b and c) Representative images of Western blot showing that the expression of DII4, NICD, Hey1, Hes1, VEGFR2, VEGF-A, NRP-1, and Tie-2 increased 14 days after transplantation of rMSCs or rMSCsHIF-2α. (d) ELISA analysis of serum Ang1. (e) ELISA analysis of serum Ang2.
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