Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2α Improves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model

Figure 5

Effect of HIF-2α overexpression on rMSC behavior. (a) HIF-2α overexpression did not affect rMSC proliferation. (b) HIF-2α overexpression increased rMSC migration under hypoxia (5% O₂) condition. The representative images of scratched cell layer were collected after 24 hours of incubation. indicates significant difference of the indicated group versus rMSCs group under the same condition, . (c) HIF-2α overexpression increased rMSC tube formation in matrigel under hypoxia (5% O₂) condition. The representative images of tube formation in matrigel were collected after 24 hours of incubation. indicates significant difference of the indicated group versus rMSCs group under the same condition, . (d) Coculture with rMSCs^HIF-2α significantly increased the invasion of endothelial cells under hypoxia condition. The representative images of invaded endothelial cells were collected after 24 hours of incubation. indicates significant difference of the indicated group versus rMSCs group under the same condition, .

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