Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression

Figure 1

Adherent, fibroblast-like morphology of MSCs at passage 0 (a) and passage 3 (b). Scale bars: 200 μm. (c) Surface marker expression on bone marrow-derived MSCs at passage 3 analyzed using flow cytometry. Black lines represent isotype control. (d–f) Differentiation potency of MSCs at passage 3. (d) Adipogenic differentiation (oil red staining, ×200). (e) Osteogenic differentiation (alizarin red staining, ×200). (f) Chondrogenic differentiation (alcian blue staining, ×200). Scale bars: (d) and (e) were 200 μm, and (f) was 100 μm.

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