Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression

Figure 3

(a) Surface marker expression on vitrified MSCs from the VD, VE, and VC groups analyzed by flow cytometry. Black lines represent isotype control. (b) Differentiation potency of MSCs from the VC, VD, and VE groups. The cells with adipogenic induction show lipid droplets identified using oil red staining; the cells with osteogenic induction show the aggregation of micronodules or calcium deposits identified using alizarin red staining; the cells with chondrogenic induction show vacuolized cells identified using alcian blue stain. Scale bars: 200 μm.

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