Mitochondrial Maturation in Human Pluripotent Stem Cell Derived Cardiomyocytes

Figure 5

Respiratory complex activities in hESC-CM (a–c), hiPSC-CM (d–f), and human heart tissue (g, h). Respiratory complex activities are measured in 100,000 cells of either hESC-CM (hESC-CM) or hiPSC-CM (d), normalized to total protein (b, e) or citrate synthase activity (c, f). Respiratory complex activities are measured in fetal hearts (50–60 days and 100–115 days), and adult heart tissue lysate, normalized to protein amount (g) or citrate synthase activity (h) of the corresponding samples. versus early CM or 50–60 d CM. adult versus 100–115 d; –5 biological replicates.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)