Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage

Figure 1

Cell viability and proliferation. Graphs show the proliferation rate at different time of each cell primary cultures at P2 and P15. Bar graphs display the exponential growth pattern of (a) hPDLSCs, (c) hDPSCs, and (e) hGMSCs, evaluated by MTT assay. Proliferation rate of (b) hPDLSCs, (d) hDPSCs, and (f) hGMSCs, performed by Trypan blue exclusion test, confirmed MTT assay results. Cells showed a logarithmic proliferation trend at P2 and P15 without any statistically significant differences. The y-axis shows cell number and x-axis shows culture time. Results in each bar graph are the composite data from experiments performed in triplicate (mean ± SEM).