Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage

Figure 3

Senescence-associated β-galactosidase staining. (a, c, and e) hPDLSCs, hDPSCs, and hGMSCs from P2 were stained with X-gal solution. (b, d, and f) hPDLSCs, hDPSCs, and hGMSCs at P15 were stained with X-gal solution and showed low levels of blue precipitation (Mag.: 10x). (b, d, and f) X-gal positive staining of hPDLSCs, hDPSCs, and hGMSCs at high magnification (Mag.: 40x). (g) Percentage of β-gal-positive cells. Results are representative of three independent experiments. Bar: 10 μm.