CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

Figure 3

CD60b is expressed along rat adult subventricular zone in proliferative and stem/progenitor cells. (a) Confocal image of a coronal section of the lateral ventricle of adult rat showing expression of CD60b in red. We can note cells expressing CD60b antigens in the lateral wall near the RMS (arrowheads) and cells CD60b-positive in the medial wall of the lateral ventricle (arrows). (b) High magnification of the SVZ showing punctiform expression of CD60b in SVZ cells along the lateral wall. We can notice that not all the cells express CD60b. (c–n) Confocal images of cells dissociated from the subventricular zone of adult rat stained with TO-PRO-3 (in blue; c, g, and k), showing the expression of CD60b (in red; (d, h, and l)) costained for BrdU (e), GFAP (i), or nestin (m) in green. Merge images are show in (f, j, and n). (o–q) Confocal Z-stack reconstruction and orthogonal slices (superior and lateral boxes) showing cells double-labeled (arrows in q) for CD60b in red (o) and nestin in green (p). Merge images showing colocalization in (q). We can also note CD60b-positive cells that do not express nestin (arrowheads in (q)). Scale bar: 300 μm (a); 30 μm (b); 12 μm (c–q). cc: corpus callosum; LV: lateral ventricle; st: striatum.

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