CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

Figure 5

CD60b is expressed in different populations in the adult SVZ. Confocal images of adult SVZ sections showing the expression of CD60b (a, d, g, j, and m in red) and doublecortin (b-c), EGFR (e-f), GFAP (h-i), PNA (k-l), and vimentin (n-o) (in green). Nuclei were counterstained with TO-PRO-3 (blue). We observed the expression of CD60b in DCX (expressed in neuroblasts), R-EGF (expressed mostly in type C cells), GFAP (expressed in type B cells and astrocytes), and vimentin-positive cells (expressed in radial glia-like cells) (arrows). PNA, which was described as a negative marker of neural stem cells, and CD60b showed little colocalization (l). High magnification is showed in the insets. LV: lateral ventricle; DCX: doublecortin. Scale bar: 20 μm.

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