Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells

Figure 1

PRGF induces proliferation and migration of human ASCs. (a) Left: Human ASCs were cultured with HS or PRGF at growing concentration (1, 2.5, 5, or 10%) or in the absent of growth factors (0%) for 24 hours and subjected to cell viability test analysis. 10% PRGF induced a significant difference on the cell numbers in comparison with 10% of HS; versus 0% and ^$ versus 10% HS; Right: representative phase-contrast images of ASCs 24 hours after incubation with 10% HS or 10% PRGF; scale bar: 100 μm. (b) Cell migration and invasion assay was performed in 96-well plates by the IncuCyte S3 live-cell analysis. Left: cell density quantification at the wound area showed a faster and significant increase of cell density induced by 10% of PRGF since 2 hours after stimulation. 10% HS significantly induced the cell migration and invasion in comparison with 0% since 10 hours of incubation. ; . Right: representative phase-contrast images of human ASCs in the presence of different concentrations of HS or PRGF 16 hours after incubation.

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