Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells

Figure 2

PRGF prevents in vitro ASC aging. (a) Senescence cell quantification was performed by quantification of SAβGal-positive cells in bright field microscope at 20x magnification. Four different fields, containing a minimum of 200 cells, were analysed in three independent experiments. (b) Autophagosome quantification was performed from higher magnification TEM pictures from at least 200 different cells. The total number of autophagosomes was normalized to the total area analyzed with ImageJ software. Three independent experiments were analysed and represented as the mean ± SD of autophagosomes/total cell area (px²) ; .

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