Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele

Figure 2

Gene expression analysis of MMC-AFSCs and healthy AFSCs. (a) Expression profile of pluripotency genes OCT4, NANOG, early neural gene PAX6, mesoendodermal genes PDGFRβ and GATA4, myogenic transcription factor PAX7, osteochondrogenic genes SOX9 and RUNX2, and COL2A1 in MMC-AFSCs and healthy AFSCs by RT-qPCR analysis. Data were represented as relative fold expression, normalized for the housekeeping gene GAPDH; NS, not significant. and values are indicated as mean ± SD. (b) Expression profile of early neural and radial glial genes SOX2, NESTIN, BLBP, and PAX3 in MMC-AFSCs and healthy AFSCs by RT-qPCR analysis. Data were represented as relative fold expression, normalized for the housekeeping gene GAPDH; and values are indicated as mean ± SD. Healthy versus MMC (NESTIN and PAX3); Healthy versus MMC (SOX2 and BLBP).

(a)

(b)